Alterations in proteins and amino acids of the Nile cyanobacteria Pseudanabaena limnetica and Anabaena wisconsinense in response to industrial wastewater pollution

Brazilian Archives of Biology and Technology

Mostafa Mohamed El-sheekh²  Ahmed Mohamed El-otify¹  Hani Saber¹ 

[1] ,Tanta University Faculty of Science Department of Botany,Egypt

关键词: amino acids;    Anabaena;    electrophoretic patterns;    Nile cyanobacteria;    protein;    Pseudanabaena;    wastewater;   

DOI  :  10.1590/S1516-89132011000400022

来源: SciELO

【 摘 要 】

The effect of industrial wastewater on the Nile cyanobacteria Pseudanabaena limnetica and Anabaena wisconsinense was investigated. The data showed that P. limnetica was more sensitive to pollution than A. wisconsinense. The treatments with different levels of wastewater exerted pronounced reductions in protein and amino acids content. SDS-PAGE analysis revealed that the cyanobacteria grown in the industrial wastewater showed induction in the synthesis of certain polypeptides and repression of others. The treatments of P. limnetica with wastewater stimulated the appearance of six protein bands with molecular masses of 28, 30, 31, 32, 58 and 97 kDa. The same treatments caused the disappearance of 20, 38 and 56 kDa. The structural protein pattern of the treated A. wisconsinense showed appearance of 16, 30, 170 and 230 kDa and disappearance of 56 kDa. The treatment of the two investigated cyanobacteria with different levels of wastewater stimulated the biosyntheses of different amino acids and inhibited others.

【 授权许可】

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