Production and characterization of poly-3-hydroxybutyrate from crude glycerol by Bacillus sphaericus NII 0838 and improving its thermal properties by blending with other polymers

Brazilian Archives of Biology and Technology

Raveendran Sindhu²  Balakrishnan Ammu²  Parameswaran Binod²  Sreelatha K. Deepthi²  K. B. Ramachandran¹  Carlos Ricardo Soccol¹  Ashok Pandey² 

[1] ,National Institute for Interdisciplinary Science and Technology Biotechnology Division ,India

关键词: polyhydroxybutyrate;    Bacillus sphaericus;    polymer blends;    crude glycerol;    biodiesel industry;   

DOI  :  10.1590/S1516-89132011000400019

来源: SciELO

【 摘 要 】

The aim of this work was to study the production of poly-3-hydroxybutyrate (PHB) under nitrogen limited conditions by Bacillus sphaericus NII 0838 using crude glycerol from biodiesel industry as sole carbon source. Effect of various process parameters on PHB production such as glycerol concentration, inoculum size and pH of the medium were optimized. Characterization of extracted PHB was carried out by FT-IR, ¹H and 13C NMR. Results showed that the bacterial culture accumulated about 31% PHB in crude glycerol medium. The extracted PHB was blended with other polymers to improve its physical characteristics. The thermal properties of the polymer like melting temperature (Tm) and heat of fusion (ΔHf) were determined using DSC.

【 授权许可】

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