Anticoagulant activity of a sulfated polysaccharide isolated from the green seaweed Caulerpa cupressoides

Brazilian Archives of Biology and Technology

José Ariévilo Gurgel Rodrigues²  Edfranck De Sousa Oliveira Vanderlei¹  Érika Façanha Bessa¹  Francisco De Araújo Magalhães¹  Regina Célia Monteiro De Paula¹  Vilma Lima¹  Norma Maria Barros Benevides¹ 

[1] ,Rede Nordeste de Biotecnologia Programa de Pós-graduação em Biotecnologia Fortaleza CE ,Brasil

关键词: marine algae;    sulfated macromolecules;    chemical analysis;    APTT test;   

DOI  :  10.1590/S1516-89132011000400007

来源: SciELO

【 摘 要 】

The aim of this study was to evaluate certain molecular characteristics of a sulfated polysaccharide (SPs) with anticoagulant properties, isolated from Caulerpa cupressoides (Chlorophyta). Crude SPs were extracted by proteolytic digestion (papain), followed by ion-exchange chromatography on a DEAE-cellulose column. The fractions obtained were analyzed for molecular mass, 0.5% agarose gel electrophoresis and chemical composition. The activated partial thromboplastin time (APTT) test was applied using normal human plasma and standard heparin (HEP) (193 IU mg-1). The yield was ~ 3%, and the chromatography procedure separated the material into three different SP fractions (F I, F II and F III), eluted at the concentrations of 0.50, 0.75 and 1.00 M of NaCl, respectively. Only fraction F II was active (24.62 IU mg-1), with high sulfate content (23.79%) and number of molecular mass peaks. Therefore, the APTT of a fraction isolated from C. cupressoides was less potent than HEP.

【 授权许可】

CC BY-NC   
 All the contents of this journal, except where otherwise noted, is licensed under a Creative Commons Attribution License