Genetic variability among the wild boars (Sus scrofa scrofa), crossbred animals and pigs using microsatellite markers (STRs)

Brazilian Archives of Biology and Technology

Paula Viana Correa Da Silva²  Jeffrey Frederico Lui²  Guilherme De Oliveira Band²  Luciana Correia De Almeida Regitano¹  Selma De Fátima Grossi¹  Bruna Pena Sollero¹  Cleujosí Da Silva Nunes¹ 

[1] ,Universidade Estadual Paulista Departamento de Zootecnia Jaboticabal SP ,Brasil

关键词: molecular genetics;    wild boars;    microsatellites;    fragments;    amplification;   

DOI  :  10.1590/S1516-89132011000200011

来源: SciELO

【 摘 要 】

The aim of this work was to study the genetic variability among the wild boars, crossbred animals and pigs using microsatellite markers. Five genetic groups were studied. The fragments of three microsatellites developed for Sus scrofa domestica - IGF1, ACTG2 and TNFB - were amplified through PCR technique to evaluate the expected intra populacion variability (He) and observed (Ho) heterozygosity, and endogamy coefficient (F IS ) within each population and inter population variability F IS , testing relationship among five genetic groups to establish the genetic distance among them. The high level of observed heterozygosity values varied between 0.537 and 0.7871. Generally, F IS was low, suggesting that the endogamy did not exist between the tested animals.

【 授权许可】

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