Biotechnological potential of alternative carbon sources for production of pectinases by Rhizopus microsporus var. rhizopodiformis

Brazilian Archives of Biology and Technology

André Ricardo De Lima Damásio²  Alexandre Maller²  Tony Márcio Da Silva¹  João Atílio Jorge¹  Hector Francisco Terenzi¹  Maria De Lourdes Teixeira De Moraes Polizeli¹ 

[1] ,Universidade de São Paulo Faculdade de Medicina de Ribeirão Preto Departamento de Bioquímica e ImunologiaRibeirão Preto SP ,Brasil

关键词: agro industrial residues;    lemon peel;    pectin lyase;    polygalacturonase;    R. microsporus var. rhizopodiformis;   

DOI  :  10.1590/S1516-89132011000100019

来源: SciELO

【 摘 要 】

Fungi collected from Brazilian soil and decomposing plants were screened for pectinase production. R. microsporus var. rhizopodiformis was the best producer and was selected to evaluate the pectic enzyme production under several nutritional and environmental conditions. The pectinase production was studied at 40ºC, under 28 carbon sources-supplemented medium. The inducer effect of several agro-industrial residues such as sugar cane bagasse, wheat flour and corncob on polygalacturonase (PG) activity was 4-, 3- and 2-fold higher than the control (pectin). In glucose-medium, a constitutive pectin lyase (PL) activity was detected. The results demonstrated that R. microsporus produced high levels of PG (57.7 U/mg) and PL (88.6 U/mg) in lemon peel-medium. PG had optimum temperature at 65 ºC and was totally stable at 55 ºC for 90 min. Half-life at 70 ºC was 68 min. These results suggested that the versatility of waste carbon sources utilization by R. microsporus, produce pectic enzymes, which could be useful to reduce production costs and environmental impacts related to the waste disposal.

【 授权许可】

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