Respirometry kinetics of phenol oxidation by Comamonas testosteroni Pb50 under various conditions of nutritional stress

Brazilian Archives of Biology and Technology

Jan Paca²  Alena Kosteckova²  Leona Pacova¹  Ales Prell¹  Martin Halecky²  Jan Paca Jr.¹  Marie Stiborova¹  Evguenii Kozliak¹  Carlos Ricardo Soccol¹ 

[1] ,Institute of Chemical TechnologyPrague,Czech Republic

关键词: Respirometry;    kinetics;    phenol;    nutrition stress;   

DOI  :  10.1590/S1516-89132010000600030

来源: SciELO

【 摘 要 】

Kinetics of phenol biodegradation using suspended biomass of Comamonas testosteroni Pb50 (monoculture) was measured under conditions of nutrient abundance, limitation, and prolonged cell starvation in a fed-batch reactor, with phenol being the sole carbon and energy source. The pre-washed cells were applied for measurement of the phenol and oxygen uptake rates at varied starting phenol concentrations with the kinetic parameters calculated using the Haldane model. The results revealed that nutrient limitation significantly suppressed the maximum value of exogenous respiration rate while the endogenous respiration rate, affinity and tolerance to phenol increased. By contrast, cell starvation resulted in a drop of both the exogenous and endogenous respiration rates by an order of magnitude.

【 授权许可】

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