Brazilian Archives of Biology and Technology | |
Response of Paenibacillus polymyxa to iron: alternations in cellular chemical composition and the production of fusaricidin type antimicrobial compounds | |
Waseem Raza1  Wu Hongsheng1  Shen Qirong1  | |
关键词: Chemical composition; Fusaricidin; Iron; Paenibacillus polymyxa; RNA; | |
DOI : 10.1590/S1516-89132010000500019 | |
来源: SciELO | |
【 摘 要 】
In this work, growth, cellular chemical composition and production of fusaricidin type antimicrobial compounds by P. polymyxa SQR-21 were compared in tryptone broth supplemented with four concentrations of iron (25, 50, 100 and 200 µM). The data revealed that the growth of P. polymyxa SQR-21 was increased by 3-8% with the increase in concentration of ferric ion (Fe3+). The production of fusaricidin type compounds was increased by 33-49% only up to 50 µM Fe3+ and the highest level of Fe3+ was inhibitory. Increase in the liquid culture Fe3+concentration increased the intracellular protein (2%), intracellular carbohydrate (14%), extracellular protein (7%) and polysaccharide contents (18%) while the intracellular lipid contents were increased (11%) only up to 50 µM Fe3+. In addition, the regulatory effects of Fe3+ were also reflected by the increase in total RNA contents and relative expression of the fusaricidin synthetase gene (FusA) by 3-13 and 35-56%, respectively, up to 50 µM Fe3+, after that a continuous decrease was observed.
【 授权许可】
CC BY-NC
All the contents of this journal, except where otherwise noted, is licensed under a Creative Commons Attribution License
【 预 览 】
Files | Size | Format | View |
---|---|---|---|
RO202005130166254ZK.pdf | 123KB | download |