Antibiosis and dark-pigments secretion by the phytopathogenic and environmental fungal species after interaction in vitro with a Bacillus subtilis isolate

Brazilian Archives of Biology and Technology

Alexandre Paulo Machado²  Viviane Karolina Vivi²  José Roberto Tavares¹  Frederico José Gueiros Filho¹  Olga Fischman¹ 

[1] ,Universidade Federal de Mato Grosso Faculdade de Ciências Médicas Departamento de Ciências Básicas em SaúdeCuiabá MT ,Brasil

关键词: Bacillus subtilis;    phytopathogenic fungi;    environmental fungi;    microbial interaction;   

DOI  :  10.1590/S1516-89132010000500001

来源: SciELO

【 摘 要 】

In this work, different reactions in vitro between an environmental bacterial isolate and fungal species were related. The Gram-positive bacteria had terminal and subterminal endospores, presented metabolic characteristics of mesophilic and acidophilic growth, halotolerance, positive to nitrate reduction and enzyme production, as caseinase and catalase. The analysis of partial sequences containing 400 to 700 bases of the 16S ribosomal RNA gene showed identity with the genus Bacillus. However, its identity as B. subtilis was confirmed after analyses of the rpoB, gyrA, and 16S rRNA near-full-length sequences. Strong inhibitory activity of environmental microorganisms, such as Penicillium sp, Aspergillus flavus, A. niger, and phytopathogens, such as Colletotrichum sp, Alternaria alternata, Fusarium solani and F. oxysporum f.sp vasinfectum, was shown on co-cultures with B. subtilis strain, particularly on Sabouraud dextrose agar (SDA) and DNase media. Red and red-ochre color pigments, probably phaeomelanins, were secreted by A. alternata and A. niger respectively after seven days of co-culture.

【 授权许可】

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