Brazilian Archives of Biology and Technology | |
Three tubulin genes of Trichoderma harzianum: alpha, beta and gamma | |
Min Li2  Qian Yang1  Jinzhu Song1  | |
[1] ,Faculty of Chemistry Biology and Material Sciences | |
关键词: Trichoderma harzianum; tubulin; codon usage bias; motif; | |
DOI : 10.1590/S1516-89132010000400009 | |
来源: SciELO | |
【 摘 要 】
Three tubulin genes of Trichoderma harzianum were cloned followed genomic walking procedure. The tubulins showed high degree of amino acid homology with other fungal tubulins and were homologous with each other with 32 to 38% amino acid identity. Three measures for the degree of codon usage bias indicated the presence of bias in all the sequences, suggesting high expression levels in all the genes. Protein structures were modeled to provide the basis for understanding the tubulin's properties and its interactions with microtubule-associated proteins. Potential motifs were also postulated.
【 授权许可】
CC BY-NC
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