Effect of deoxynivalenol and T-2 toxin in malt amylase activity

Brazilian Archives of Biology and Technology

Jaqueline Garda-buffon¹  Edlira Baraj²  Eliana Badiale-furlong² 

[1] ,Fundação Universidade Federal do Rio Grande Departamento de Química Laboratório de MicotoxinasRio Grande do Sul RS ,Brasil

关键词: Aminolitic enzymes;    fermentation;    trichothecenes;    barley;   

DOI  :  10.1590/S1516-89132010000300002

来源: SciELO

【 摘 要 】

The objective of this work was to evaluate the effect of DON and T-2 toxin levels on the malt amylase activity. The malt was contaminated in accordance with central composite design experiment with DON and T-2 toxin levels until 1000 ng/g. The activities of the enzymes were evaluated by Bernfeld method. The increase in T-2 toxin concentration inhibited the α-amylase activity. However, the increase of both toxins concentration caused inverse effect. The interaction between toxins indicated synergetic effect on the β-amylase activity. An increased activity occurred when the toxins contamination levels in malt were higher (1000ng/g malt). The trichothecenes interfered with the performance of aminolitic enzymes in the stage of malting, resulting in a significant model for enzymatic activity of β-amylase.

【 授权许可】

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