Kinetic analysis of in vitro production of wild-type Spodoptera frugiperda nucleopolyhedrovirus

Brazilian Archives of Biology and Technology

Andréa Farias De Almeida¹  Gorete Ribeiro De Macedo¹  Leslie Chee Loong Chan²  Márcia Regina Da Silva Pedrini² 

[1] ,Universidade Federal do Rio Grande do Norte Departamento de Engenharia Química Natal,Brasil

关键词: Baculovirus;    in vitro production;    kinetic analysis;    SfMNPV;   

DOI  :  10.1590/S1516-89132010000200006

来源: SciELO

【 摘 要 】

In this study, the kinetic behavior of Sf9 and Sf21 cells used in the production of a baculovirus biopesticide to control the pest of corn Spodoptera frugiperda was analyzed. Kinetic variables such as maximum specific growth rate, cell productivity, mean rate of infection, as well as the mean rate of occlusion body production were determined during the infection of these cell-lines with the extracellular virus of the S. frugiperda nucleopolyhedrovirus (SfMNPV). The Sf9 cell-line resulted in better viral production results (5.0 x 10(8) OB/mL) than the Sf21 cell-line (2.5 x 10(8) OB/mL).

【 授权许可】

CC BY-NC   
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