Use of oval conidia as a tool to assess the genetic transfer among Colletotrichum sublineolum mutants

Brazilian Archives of Biology and Technology

Maria Paula Nunes¹  Ednéia Aparecida De Souza-paccola¹  Gisele Maria Andrade-nóbrega¹  Luzia Doretto Paccola-meirelles¹ 

[1] ,Universidade Estadual de Londrina Departamento de Biologia Geral Laboratório de Genética de FungosLondrina PR ,Brasil

关键词: genetic recombination;    nit mutants;    parameiosis;   

DOI  :  10.1590/S1516-89132010000100021

来源: SciELO

【 摘 要 】

The aim of this study was to assess oval conidia production in mutants for the reductase nitrate gene in C. sublineolum and verify the possibility of using them as a tool to transfer the genetic material in this species. The mutants used in the present study lost the ability to form falcate conidia but all produced oval conidia. The number of nuclei by conidium was evaluated. Oval conidia were efficient in the heterokaryon formation and these heterokaryons in liquid culture medium they also produced oval conidia in abundance. Recombinants were obtained and the genetic exchanges were confirmed by the RAPD analyses.

【 授权许可】

CC BY-NC   
 All the contents of this journal, except where otherwise noted, is licensed under a Creative Commons Attribution License