Quantitative differential expression of alpha and beta ryanodine receptor genes in PSE (Pale, Soft, Exudative) meat from two chicken lines: broiler and layer

Brazilian Archives of Biology and Technology

Sandra Helena Inoue Oda²  Alexandre Lima Nepomuceno¹  Mônica Corrêa Ledur¹  Maria Cristina Neves De Oliveira¹  Silvana Regina Rockenbach Marin¹  Elza Iouko Ida²  Massami Shimokomaki² 

[1] ,Universidade Estadual de Londrina Centro de Ciências Agrárias Departamento de Ciências e Tecnologia de AlimentosLondrina PR ,Brasil

关键词: malignant hyperthermia;    poultry;    gene expression;    real time PCR;   

DOI  :  10.1590/S1516-89132009000600024

来源: SciELO

【 摘 要 】

Total RNA isolated from Pectoralis major muscle from PSE (L*24h>53.0, pH<5.8) and non-PSE (44<"L*24h>"53) meats of two phenotypically distinct chicken lines, broiler and layer, was used to investigate the α-ryr and β-ryr gene expression by real-time RT-PCR approach. Mean relative quantification (RQ) values were lower (p<0.05) for β-ryr in PSE chickens from both lines when compared to non-PSE chickens, while there was no difference (p>0.05) in α-ryr gene expression regardless of line studied. The β-ryr RQ results suggested that in PSE samples an alteration might occur in the regular ratio (1:1) of α-RyR/β-RyR normally found in avian muscles. These results provided the first evidence of PSE meat occurrence as a result of the differential expression of ryanodine receptor genes which might lead to an increased in Ca2+ availability at the cell milieu.

【 授权许可】

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