Selective agent and A. tumefaciens overgrowth-control antibiotics in Eucalyptus camaldulensis Cotiledonary culture

Brazilian Archives of Biology and Technology

Regina Quisen²  Yohana De Oliveira¹  Marcos Pileggi¹  Francine Cuquel¹  Marguerite Quoirin¹ 

[1] ,Embrapa Amazônia OcidentalManaus AM ,Brasil

关键词: Morphogenesis;    kanamycin;    cefotaxime;    augmentin;    Agrobacterium tumefaciens;    eucalypt;   

DOI  :  10.1590/S1516-89132009000600020

来源: SciELO

【 摘 要 】

The objectives of the present work were to establish the minimal lethal dose of the selective agent to determine the type and concentration of appropriate antibiotics for the elimination of Agrobacterium tumefaciens inoculated explants, without interfering with the regenerative potential of the E. camaldulensis cotyledonary explants. Non-transformed explants were cultivated in medium supplemented with kanamycin. The results showed that the antibiotic was suitable for the selection of transformed cells in the concentration of 9 mg L-1 as it inhibited the growth of non-transformed cells. Cotyledons infected with A. tumefaciens were cultivated in MS N/2 medium supplemented with BAP, ANA, Km and cefotaxime or AugmentinÒ . The highest average of regenerated shoots by explant (5,4) was observed in the presence of 300 mg L-1 of AugmentinÒ /15 days, followed by 150 mg L-1/15 days and 100 mg L-1/30 days.

【 授权许可】

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