Investigation about the efficiency of the bioaugmentation technique when applied to diesel oil contaminated soils

Brazilian Archives of Biology and Technology

Adriano Pinto Mariano²  Dejanira De Franceschi De Angelis¹  Maria Paula Santos Pirôllo¹  Jonas Contiero¹  Daniel Marcos Bonotto² 

[1] ,Universidade Estadual Paulista Instituto de Geociências e Ciências Exatas Rio Claro SP ,Brasil

关键词: bioremediation;    bioaugmentation;    soil;    diesel oil;    service station;   

DOI  :  10.1590/S1516-89132009000500030

来源: SciELO

【 摘 要 】

This work investigated the efficiency of the bioaugmentation technique when applied to diesel oil contaminated soils collected at three service stations. Batch biodegradation experiments were carried out in Bartha biometer flasks (250 mL) used to measure the microbial CO2 production. Biodegradation efficiency was also measured by quantifying the concentration of hydrocarbons. In addition to the biodegradation experiments, the capability of the studied cultures and the native microorganisms to biodegrade the diesel oil purchased from a local service station, was verified using a technique based on the redox indicator 2,6 -dichlorophenol indophenol (DCPIP). Results obtained with this test showed that the inocula used in the biodegradation experiments were able to degrade the diesel oil and the tests carried out with the native microorganisms indicated that these soils had a microbiota adapted to degrade the hydrocarbons. In general, no gain was obtained with the addition of microorganisms or even negative effects were observed in the biodegradation experiments.

【 授权许可】

CC BY-NC   
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