An efficient and rapid DNA minipreparation procedure suitable for PCR/SSR and RAPD analyses in tropical forest tree species

Brazilian Archives of Biology and Technology

Ana Lilia Alzate-marin²  Marcela Corbo Guidugli²  Hilda Hildebrand Soriani²  Carlos Alberto Martinez¹  Moacyr Antônio Mestriner² 

[1] ,Faculdade de Medicina de Ribeirão Preto Departamento de Genética Laboratório de Genética VegetalRiberão Preto SP ,Brasil

关键词: Copaifera langsdorffii;    Hymenaea courbaril;    Eugenia uniflora;    Tabebuia roseo alba;    Cariniana estrellensis;    DNA extraction;   

DOI  :  10.1590/S1516-89132009000500020

来源: SciELO

【 摘 要 】

An efficient and rapid DNA minipreparation modified method for frozen samples was developed for five tropical tree species: Copaifera langsdorffii, Hymenaea courbaril, Eugenia uniflora, Tabebuia roseo alba and Cariniana estrellensis. This procedure that dispenses the use of liquid nitrogen, phenol and the addition of proteinase K, is an adaptation of the CTAB-based DNA extraction method. The modifications included the use of PVP to eliminate the polyphenols, only one chloroform-isoamyl alcohol step and the addition of RNase immediately after extraction with chloroform. The yields of the DNA samples ranged from 25.7 to 42.1 µg from 100 mg leaf tissue. The DNA samples extracted by this method were successfully used for PCR (SSR and RAPD) analyses in these five and other twelve tropical tree species.

【 授权许可】

CC BY-NC   
 All the contents of this journal, except where otherwise noted, is licensed under a Creative Commons Attribution License