Influence of pH on the growth, laccase activity and RBBR decolorization by tropical basidiomycetes

Brazilian Archives of Biology and Technology

Sérgio Luiz Moreira Neto²  Dácio Roberto Matheus¹  Kátia Maria Gomes Machado² 

[1] ,Universidade Católica de Santos Departamento de Ciências Biológicas Santos SP ,Brasil

关键词: White rot fungi;    RBBR;    laccase;    peroxidases;   

DOI  :  10.1590/S1516-89132009000500003

来源: SciELO

【 摘 要 】

The basidiomycete fungi Lentinus crinitus and Psilocybe castanella are being evaluated in a bioremediation process of soils contaminated with organochlorine industrial residues in the Baixada Santista, São Paulo. The aim of the present study was to determine the influence of pH on the fungal growth, in vitro decolorization of anthraquinonic dye Remazol Brilliant Blue R (RBBR) and laccase activity. The pH of the culture medium influenced the growth of L. crinitus and P. castanella, which presented less growth at pH 5.9 and pH 2.7, respectively. The fungi were able to modify the pH of the culture medium, adjusting it to the optimum pH for growth which was close to 4.5. Decolorization of the RBBR was maximal at a pH of 2.5 to 3.5. Higher laccase activity was observed at pH 3.5 and pH 4.5 for L. crinitus and P. castanella, respectively. pH was found to be an important parameter for both the growth of these fungi and the enzymatic system involved in RBBR decolorization.

【 授权许可】

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