High-yield production of scutellaria radix flavonoids (baicalein, baicalin and wogonin) by liquid-culture of Scutellaria baicalensis root-derived cells

Brazilian Archives of Biology and Technology

Takashi Ohtsuki²  Mika Himeji²  Harumi Fukazawa²  Miho Tanaka²  Hisako Yamamoto¹  Akio Mimura² 

[1] ,University of Yamanashi Faculty of Engineering Department of BiotechnologyJapan

关键词: Antioxidant;    flavonoids;    liquid culture;    high-yield production;    Scutellaria baicalensis;   

DOI  :  10.1590/S1516-89132009000200005

来源: SciELO

【 摘 要 】

Production of baicalein, baicalin and wogonin by liquid culture of Scutellaria baicalensis cells derived from the plant root was studied. The maximum production obtained were 119 mg/L of baicalein at two week, 1372 mg/L of baicalin at eight week, and 14 mg/L of wogonin at two week. In addition, the production of baicalin was drastically increased to 1000 mg/L level at 3-week culture, and the extremely high production rate (339 mg/L•week) was obtained. In the comparison of total antioxidative activities among baicalein, baicalin and wogonin, evaluated by thiocyanate method, it was suggested that the location of hydroxyl groups both at 5- and 6-position contributed to enhancement of radical scavenging activity, and/or methoxylation at 8-position diminished the activity. The possibility of utilizing these flavonoids for natural antioxidants and medicine is also discussed.

【 授权许可】

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