Cloning of high molecular weight gluten subunit promoter and study on its function in wheat

Brazilian Archives of Biology and Technology

Jin Weibo¹  Liu Jin¹  Wu Fangli¹  Guo Aiguang¹ 

[1] ,Northwest A & F University Yangling College of Life Science Shaanxi,China

关键词: Triticum aestivum;    glutenin;    promoter;    endosperm-specific;    transient expression;   

DOI  :  10.1590/S1516-89132009000200002

来源: SciELO

【 摘 要 】

The aim of this work was to study the cloning and characterization of HMW-GS 1Dx2 promoter from Triticum aestivum. A 1050 bp partial promoter fragment including a putative TATA box and 5' encoding sequence of the gene was cloned by amplifying the upstream sequences using the nest-PCR with appropriate primers. The analysis of the promoter sequence against the PLACE (Plant cis-acting Regulatory DNA Elements) database showed the presence of certain putative endosperm-specific regulatory cis-elements in the sequence along with the TATA and CAAT boxes. The histochemical method detected the transient expressions of GUS in the seeds of wheat. The results showed that HMW-GS 1Dx2 promoter had the endosperm-specific transcription activity in the wheat seeds.

【 授权许可】

CC BY-NC   
 All the contents of this journal, except where otherwise noted, is licensed under a Creative Commons Attribution License