Adsorption of amyloglucosidase from Aspergillus niger NRRL 3122 using ion exchange resin

Brazilian Archives of Biology and Technology

Ana Paula Manera²  Eliana Setsuko Kamimura¹  Luciana Machado Brites²  Susana Juliano Kalil² 

[1] ,Universidade Federal do Rio Grande Departamento de Química Rio Grande RS ,Brasil

关键词: amyloglucosidase;    adsorption isotherm;    kinetics adsorption;    ion exchange resin;    purification;   

DOI  :  10.1590/S1516-89132008000500019

来源: SciELO

【 摘 要 】

Amyloglucosidase enzyme was produced by Aspergillus niger NRRL 3122 from solid-state fermentation, using deffated rice bran as substrate. The effects of process parameters (pH, temperature) in the equilibrium partition coefficient for the system amyloglucosidase - resin DEAE-cellulose were investigated, aiming at obtaining the optimum conditions for a subsequent purification process. The highest partition coefficients were obtained using 0.025M Tris-HCl buffer, pH 8.0 and 25ºC. The conditions that supplied the highest partition coefficient were specified, the isotherm that better described the amyloglucosidase process of adsorption obtained. It was observed that the adsorption could be well described by Langmuir equation and the values of Qm and Kd estimated at 133.0 U mL-1 and 15.4 U mL-1, respectively. From the adjustment of the kinetic curves using the fourth-order Runge-Kutta algorithm, the adsorption (k1) and desorption (k2) constants were obtained through optimization by the least square procedure, and the values calculated were 2.4x10-3 mL U-1 min-1 for k1 and 0.037 min-1 for k2 .

【 授权许可】

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