Sequence and analysis of the mitochondrial DNA control region in the sugarcane borer Diatraea saccharalis (Lepidoptera: Crambidae)

Brazilian Archives of Biology and Technology

Juliana Pereira Bravo¹  Joice Felipes¹  Daniela Bertolini Zanatta¹  José Luis Da Conceição Silva¹  Maria Aparecida Fernandez¹ 

[1] ,UEM Departamento de Biologia Celular e Genética Maringá PR ,Brasil

关键词: mtDNA Control Region;    Diatraea saccharalis;    Lepidoptera;    sugarcane borer;   

DOI  :  10.1590/S1516-89132008000400004

来源: SciELO

【 摘 要 】

This study aimed at the sequence and analysis of the mtDNA control region (CR) of the Diatraea saccharalis. The genome PCR amplification was performed using the complementary primers to the flanking regions of Bombyx mori CR mitochondrial segment. The sequencing revealed that the amplified product was 568 bp long, which was smaller than that observed for B. mori (725 bp). Within the amplified segment, a sequence with 338 nucleotides was identified as the control region, which displayed a high AT content (93.5%). The D. saccharalis mtDNA CR multiple sequence alignment analysis showed that this region had high similarity with the Lepidoptera Cydia pomonella.

【 授权许可】

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