Comparative analysis of genetic diversity among the maize inbred lines (Zea mays L.) obtained by RAPD and SSR markers

Brazilian Archives of Biology and Technology

Silvia Graciele Hülse De Souza²  Valéria Carpentieri-pípolo²  Claudete De Fátima Ruas¹  Valdemar De Paula Carvalho¹  Paulo Maurício Ruas¹  Antônio Carlos Gerage¹ 

[1] ,Universidade Estadual de Londrina Departamento de Agronomia Londrina PR ,Brasil

关键词: Corn;    distance genetic;    microsatellites;    molecular markers;    polymorphism;    RAPD;   

DOI  :  10.1590/S1516-89132008000100022

来源: SciELO

【 摘 要 】

The RAPD and SSR markers were used to compare the genetic diversity among the 16 maize inbred lines. Twenty-two primers were used in the RAPD reactions, resulting in the amplification of 265 fragments, while 16 pairs of SSR primers resulted in 75 fragments. The similarity based on Dice coefficient for the RAPD ranged from 53 to 84% and for the SSR from 11 to 82%. The dendrogram obtained by the RAPD showed five groups, while dendrogram obtained by the SSR showed three groups and one isolated line. The association constructed from the markers and the principal coordinate’s analysis separated lines into two groups according to endosperm color, either orange or yellow. The RAPD were effective to validate pedigree data, while the SSR were effective to recognize the differences between the quantitative characters. Because they assess the distinct regions of the genome, the selection of one or other marker would depend on the characteristics of the material used and the objectives of the project.

【 授权许可】

CC BY-NC   
 All the contents of this journal, except where otherwise noted, is licensed under a Creative Commons Attribution License