Enhanced production of manganese peroxidase by Phanerochaete chrysosporium

Brazilian Archives of Biology and Technology

Raziye Ozturk Urek²  Nurdan Kasikara Pazarlioglu¹ 

[1] ,Dokuz Eylul University Faculty of Education Department of ChemistryBuca Izmir ,Turkey

关键词: Phanerochaete chrysosporium;    manganese dependent peroxidase;    Tween 80;    Mn2+;    phenylmethylsulphonylfloride;   

DOI  :  10.1590/S1516-89132007000700001

来源: SciELO

【 摘 要 】

Production of manganese-dependent peroxidase (MnP) by the white-rot fungus Phanerochaete chrysosporium BKM-F-1767 (ATCC 24725) was monitored during growth in different media and growth conditions. The effect of some activators of MnP production, Mn2+, Tween 80, phenylmethylsulphonylfloride (PMSF), oxygen, temperature, pH, glycerol and nitrogen was studied. Supplementing the cultures with Tween 80 (0.05 %, v/v) and Mn2+ (174 µM) resulted a maximum MnP activity of 356 U/L which was approximately two times higher than that obtained in the control culture (without Tween 80). Decolourisation of Direct Blue 15 and Direct Green 6 (50 mg/L) was also achieved with MnP.

【 授权许可】

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