Technetium-99m-labeled stealth pH-sensitive liposomes: a new strategy to identify infection in experimental model

Brazilian Archives of Biology and Technology

Vildete Aparecida Sousa Carmo²  Mônica Cristina De Oliveira²  Luciene Das Graças Mota¹  Luís Paulo Freire¹  Raphael Ligório Benedito Ferreira¹  Valbert Nascimento Cardoso¹ 

[1] ,Universidade Federal de Minas Gerais Faculdade de Farmácia Departamento de Produtos Farmacêuticos

关键词: 99mTechnetium;    pH-sensitive liposomes;    infection imaging;    scintigraphy;   

DOI  :  10.1590/S1516-89132007000600025

来源: SciELO

【 摘 要 】

The diagnosis of inflammatory and infectious processes is an important goal in medicine. The use of radiopharmaceuticals for identification of inflammation and infection foci has received considerable attention. The aim of this work was to evaluate the uptake and the imaging potential of stealth pH-sensitive liposomes radiolabelled with 99mTechnetium (99mTc) to identify infection sites in mice. The liposomes containing glutathione were labeled with 99mTc-Hexamethylpropyleneamine oxime (HMPAO) complex. The 99mTc-labeled stealth pH-sensitive liposomes (99mTc-SpHL) were injected in mice bearing infection in the right thigh muscle induced by Staphylococcus aureus. Biodistribution studies and scintigraphic imaging were performed at different times after injection of radiopharmaceutical. The 99mTc-SpHL was significantly uptaken by abscess when compared to the respective control. The abscess was visualized as early as 0.5 hours after injection of 99mTc-SpHL becoming more prominent with the time. These results indicate that 99mTc-SpHL is a promising radiopharmaceutical for visualizing infection foci in patients.

【 授权许可】

CC BY-NC   
 All the contents of this journal, except where otherwise noted, is licensed under a Creative Commons Attribution License