Investigation of toxic factors affecting cells of rat brains exposed to 3-methylcatechol

Brazilian Archives of Biology and Technology

George Emílio Sampaio Barreto²  Elineusa Silva De Oliveira²  Flávio Augusto Guerreiro Aragão De Villar²  Gleide Souza Dos Santos²  Rute Maria Ferreira Lima²  Silvia Lima Costa²  Maria De Fátima Dias Costa²  Eryvaldo Sócrates Tabosa Egito¹  Ramon Santos El-bachá² 

[1] ,Universidade Federal da Bahia Instituto de Ciências da Saúde Departamento de BiofunçãoSalvador Bahia ,Brasil

关键词: Astrocytes;    brain;    cytotoxicity;    3-methylcatechol;    peroxidation;    respiration;   

DOI  :  10.1590/S1516-89132007000500012

来源: SciELO

【 摘 要 】

The aim of this work was to study the effects of 3MC on the peroxidation of biomolecules in nuclear fractions and nonsynaptic mitochondrial respiration in organelles obtained from rat brains. The cytotoxicity towards rat primary astrocytes in vitro was also tested. 3MC at 1mM oxidized consuming oxygen at a rate of 1.98 ± 0.19 µM.min-1 and formed reactive quinones. At the same concentration, 3MC induced peroxidation of biomolecules in nuclear fractions obtained from rat brain homogenates and inhibited state 2 FADH2-linked respiration in nonsynaptic mitochondria. Furthermore, 3MC oxidized in the culture medium, leading to the formation of quinones. This toluene metabolite was cytotoxic to rat primary astrocytes. The concentration that killed 50% of cells after 72 h was 107 mM. The results of the study indicated a direct relationship between cytotoxicity and 3MC oxidation.

【 授权许可】

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