Penicillium expansum versus antagonist yeasts and patulin degradation in vitro

Brazilian Archives of Biology and Technology

Alexandre Rodrigo Coelho¹  Marcos Giovani Celli¹  Elisabete Yurie Sataque Ono²  Gilvan Wosiacki¹  Fernando Leite Hoffmann¹  Fernando Carlos Pagnocca¹  Elisa Yoko Hirooka¹ 

[1] ,State University of Londrina Department of Biochemistry and Biotechnology Londrina Parana ,Brazil

关键词: Penicillium expansum;    antagonist yeasts;    biocontrol;    patulin;    degradation;   

DOI  :  10.1590/S1516-89132007000400019

来源: SciELO

【 摘 要 】

Taking into account the preliminary antagonistic/biodegradation property showed by Pichia membranifaciens and Sporobolomyces roseus, which decreased the initial patulin concentration of 588.4 to 290.0 µg/mL, ability of P. ohmeri 158 in biocontrol against Penicillium expansum and patulin decrease in vitro was performed. The culture supernatant of P. ohmeri 158 was effective against 66.17% micelial growth, indicating antibiosis related with the killer phenomenon. The initial patulin concentration of 223 µg in the presence of P. ohmeri 158 cells was decreased over 83% of the original concentration, when incubated at 25ºC/2 days and > 99% after 5 days incubation time, with undetectable patulin level after 15 days. The initial pH 4.0 decreased to pH 3.3 along 15 days experiment, suggesting that patulin decrease was an active process and a consequence of yeast metabolism. The results suggested that P. ohmeri 158 could be a promising alternative for the inhibition of P. expansum growth and patulin degradation.

【 授权许可】

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