Use of chitosan gel for the purification of protein

Brazilian Archives of Biology and Technology

Zhenxing Tang²  Junqing Qian¹ 

[1] ,Zhejiang University of Technology College of Chemical Engineering and Materials Science Hangzhou Zhejiang ,China

关键词: Chitosan;    Gel;    Preparation;    Application;   

DOI  :  10.1590/S1516-89132007000200015

来源: SciELO

【 摘 要 】

The aim of this study was to optimize the preparation of chitosan gel and to use it for protein purification. The optimized preparation parameters were chitosan concentration 2.0%, glutaraldehyde concentration 0.6%, low cross-linking rate, NaOH concentration 1.6%, amount of NaBH4 0.4 g. In order to use the chitosan gel, the elution conditions were optimized as follows. NaCl concentration 0.05 mol/L in the tris-HCl (pH 9.05) at the flow rate of 2.03.0 mL/min. Particle size of chitosan gel was 120-140 µm. Neutral protease could be separated into four ingredients through chitosan gel column. The yield of enzyme was more than 90%. Albumin bovine serum could be separated into two ingredients through gel column and the total yield of albumin bovine serum was more than 70%.

【 授权许可】

CC BY-NC   
 All the contents of this journal, except where otherwise noted, is licensed under a Creative Commons Attribution License