In Vitro study of Vellozia pusilla pohl (Velloziaceae), a Brazilian plant species: antitumoral activity and labeling of blood elements

Brazilian Archives of Biology and Technology

Ana Letícia Almeida Dantas¹  Lígia Maria Marino Valente²  Lilia Aparecida Salgado De Morais²  Glaucio Feliciano²  Mário Bernardo-filho² 

[1] ,CNEN IRD Instituto de Radioproteção e Dosimetria

关键词: Drug interaction;    antitumoral activity;    technetium-99m;    blood elements;    Velloziaceae;   

DOI  :  10.1590/S1516-89132005000700008

来源: SciELO

【 摘 要 】

Vellozia pusilla Pohl is a species of the botanic family Velloziaceae that occurs in the subtropical regions of South America and, although it lives under conditions of high solar irradiation and low water availability, shows great longevity. The methanol extract of roots, stem and leaf sheaths of this species showed an antitumoral activity through the inhibition of the enzyme Topoisomerase I when analyzed by an in vitro bioassay employing DNA repair or recombination deficient mutants of the yeast Saccharomyces cerevisiae. In the evaluation of the effect of Vellozia pusilla methanol extract on the labeling of RBC, blood of mice was treated with 99mTc tracer solutions. The percentage of radioactivity (%ATI) bound to plasma (P) and blood cells (BC) was determined. The %ATI in the insoluble fraction of plasma (IF) was also evaluate, and the results showed that there was a decrease in %ATI in this fraction that represents the plasmatic proteins.

【 授权许可】

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