Preparation of crotalus venom radiolabeled with technetium-99m as a tool for biodistribution study

Brazilian Archives of Biology and Technology

Priscilla Brunelli Pujatti²  Carlos Jorge Rodrigues Simal¹  Raquel Gouvêa Dos Santos² 

[1] ,Centro de Desenvolvimento da Tecnologia Nuclear Comissão Nacional de Energia Nuclear Belo Horizonte MG ,Brasil

关键词: Technetium-99m;    labeling;    hemolytic activity;   

DOI  :  10.1590/S1516-89132005000700002

来源: SciELO

【 摘 要 】

Technetium-99m (99mTc) has been the radionuclide of choice for nuclear medicine procedures and experimental research. Because of its optimal nuclear properties, 99mTc is suitable for high efficiency detection with the advantage of reduced radiological waste. Crotalus venom (CV) has been shown to reduce tumors in clinical studies and tissue distribution studies are very important for clinical use. The goal of this work was to obtain CV labeled with 99mTc which preserves its biological activity. After labeling, biological activity was assessed by hemolytic activity evaluation. Labeled and crude venom caused indirect hemolysis provided that the incubation medium contained an exogenous source of lecithin. High yield radiolabeled-CV was obtained and biological activity was preserved. The results suggest that 99mTc-CV can be a useful tool for biodistribution studies.

【 授权许可】

CC BY-NC   
 All the contents of this journal, except where otherwise noted, is licensed under a Creative Commons Attribution License