Performance evaluation of CHO-K1 cell in culture medium supplemented with hemolymph

Brazilian Archives of Biology and Technology

Tássia Raffoul²  Kamilla Swiech²  Mabel Karina Arantes²  Álvaro Paiva Braga De Sousa¹  Ronaldo Zucatelli Mendonça¹  Carlos Augusto Pereira¹  Cláudio Alberto Torres Suazo² 

[1] ,Universidade Federal de São Carlos Departmento de Engenharia Química São Carlos SP ,Brasil

关键词: Animal cell;    CHO-K1;    microcarrier;    growth;    attachment;    hemolymph;   

DOI  :  10.1590/S1516-89132005000400011

来源: SciELO

【 摘 要 】

The aim of this work was to evaluate the potential of hemolymph utilization as a culture medium supplement to cultivate the animal cell CHO-K1. For this purpose 1% v/v of hemolymph was added to DMEM medium containing 10% v/v of FBS and 1 or 4.5 g/L of glucose. The culture was grown in spinner flasks incubated in a 10% v/v CO2 environment, at 37ºC, with the Cytodex 1 microcarrier. Comparing the results obtained from the culture with hemolymph against those without hemolymph, a positive influence of the hemolymph was observed, as the experiment with hemolymph presented a 52% higher cell concentration and a higher productivity of up to 40%.

【 授权许可】

CC BY-NC   
 All the contents of this journal, except where otherwise noted, is licensed under a Creative Commons Attribution License