Lipase production by solid-state fermentation in fixed-bed bioreactors

Brazilian Archives of Biology and Technology

Elisa D'avila Costa Cavalcanti²  Melissa Limoeiro Estrada Gutarra²  Denise Maria Guimarães Freire²  Leda Dos Reis Castilho¹  Geraldo Lippel Sant'anna Júnior¹ 

[1] ,Instituto de Química

关键词: Solid-state fermentation;    fixed-bed bioreactors;    lipase;    Penicillium simplicissimum;   

DOI  :  10.1590/S1516-89132005000400010

来源: SciELO

【 摘 要 】

In the present work, packed bed bioreactors were employed with the aim of increasing productivity and scaling up of lipase production using Penicillium simplicissimum in solid-state fermentation. The influence of temperature and air flow rate on enzyme production was evaluated employing statistical experimental design, and an empirical model was adjusted to the experimental data. It was shown that higher lipase activities could be achieved at lower temperatures and higher air flow rates. The maximum lipase activity (26.4 U/g) was obtained at the temperature of 27°C and air flow rate of 0.8 L/min.

【 授权许可】

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