Recovery of Bacillus sphaericus spores by flocculation/sedimentation and flotation

Brazilian Archives of Biology and Technology

Christine Lamenha Luna²  Carlos Edison Lopes¹  Giulio Massarani² 

[1] ,Cidade Universitária Universidade Federal do Rio de Janeiro COPPERio de Janeiro RJ ,Brasil

关键词: Bacillus sphaericus;    flocculation;    sedimentation;    flotation;   

DOI  :  10.1590/S1516-89132005000400008

来源: SciELO

【 摘 要 】

The aim of this work was use flocculation/sedimentation and flotation for recovery of spores of the Bacillus sphaericus. Microorganism was produced batchwise using culture medium based skimmed milk, corn steep liquor and mineral salts. The best results of flocculation were obtained using CaCl2.2H2O, FeCl3.6H2O, Al2(SO4)3 and tannin as flocculating agents, with optimal flocculation concentrations of 1,500, 3,000, 2,000 and 1,700ppm, respectively. Flocculent suspensions were characterized based on floc diameter and density. Settling tests were performed in batch at different concentrations of the cellular suspensions and revealed high recovery of the solids in suspension in all cases. Flotation tests were accomplished using a mechanical agitated flotation cell and the process was favoured by the increase of the system agitation and for the presence of a cationic collector.

【 授权许可】

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