Partial purification and properties of cellulase-free alkaline xylanase produced by Rhizopus stolonifer in solid-state fermentation

Brazilian Archives of Biology and Technology

Antonio José Goulart²  Eleonora Cano Carmona¹  Rubens Monti² 

[1] ,Universidade Estadual Paulista ‘Júlio de Mesquita Filho’ Faculdade de Ciências Farmacêuticas Departamento de Alimentos e NutriçãoAraraquara SP ,Brazil

关键词: alkaline xylanase;    Rhizopus stolonifer;    solid-state fermentation;   

DOI  :  10.1590/S1516-89132005000300001

来源: SciELO

【 摘 要 】

Rhizopus stolonifer was cultivated in wheat bran to produce a cellulase-free alkaline xylanase. The purified enzyme obtained after molecular exclusion chromatography in Sephacryl S-200 HR showed optimum temperature as 45º C and hydrolysis pHs optima as pH 6.0 and 9.0. Xylanase presented higher Vmax at pH 9.0 (0.87 µmol/mg protein) than at pH 6.0 and minor Km at pH 6.0 (7.42 mg/mL) than at pH 9.0.

【 授权许可】

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