Colorimetric determination of alpha and beta-cyclodextrins and studies on optimization of CGTase production from B. firmus using factorial designs

Brazilian Archives of Biology and Technology

Ilma Hiroko Higuti²  Priscila Anunciação Da Silva¹  Juliana Papp¹  Vivian Mayumi De Eiróz Okiyama¹  Edicléia Alves De Andrade¹  Aluizio De Abreu Marcondes¹  Aguinaldo José Do Nascimento¹ 

[1] ,Universidade Federal do Paraná Setor de Ciências Biológicas Departamento de Patologia Básica

关键词: Cyclodextrin glycosyltransferase;    Bacillus firmus;    cyclodextrins;   

DOI  :  10.1590/S1516-89132004000600001

来源: SciELO

【 摘 要 】

Cyclodextrin glycosyltransferase (EC 2.4.1.19, CGTase) production from B. firmus, isolated from soil of Curitiba, PR, was optimized in shake flask using an experimental design approach. The CGTase was produced when the carbon source was starch and beta-CD, but when simple sugars such as glucose, galactose, lactose, sucrose, and maltose were used, there was no enzyme production. CGTase production was the same with either organic nitrogen or inorganic nitrogen source. CGTase activity decreased 2-fold when incubation temperature was increased from 28 to 37 ° C, and decreased 2.1- fold when the initial pH was lowered from 10.3 to 7.4. The colorimetric determinations of alpha - and beta -CD were analyzed as a non-linear relationship and the equilibrium constant for alpha -CD/methyl orange and beta -CD/phenolphthalein complexes were 7.69 x 10³ L / mol and 2.33 x 10³ L/ mol, respectively.

【 授权许可】

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