Initial segmentation patterns of microspores and pollen viability in soybean cultured anthers: indication of chromosome doubling

Brazilian Archives of Biology and Technology

Milena Barcelos Cardoso²  Eliane Kaltchuk-santos²  Elsa Cristina De Mundstock¹  Maria Helena Bodanese-zanettini² 

[1] ,Universidade Federal do Rio Grande do Sul Departamento de Genética Porto Alegre RS ,Brazil

关键词: Glycine max;    FDA;    binucleate pollen grains;    multinucleate pollen grains;    spontaneous diploidization;   

DOI  :  10.1590/S1516-89132004000500005

来源: SciELO

【 摘 要 】

Anthers obtained from flowers buds of soybean cultivar IAS-5 were cultured in two basal culture media (B5 and B5 long). Cytological examinations of the in vitro anthers were performed during the first 20 days of culture to assay the viability (by propionic-carmine and fluorescein diacetate tests) and the stage of development of pollen grains. The frequencies of viable pollen grains varied significantly between bud sizes on the propionic-carmine analysis. The basal culture media and bud size had no clear effect on the frequencies of binucleate symmetrical and multinucleate pollen grains. Chromosome counts of metaphasic microspores throughout the culture period showed microspores with higher ploidy level in addition to normal chromosome number (n=20).

【 授权许可】

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