Brazilian Archives of Biology and Technology | |
Effect of temperature shock on soybean microspore embryogenesis | |
Ana Paula De Moraes2  Maria Helena Bonadese-zanettini2  Sídia Maria Callegari-jacques1  Eliane Kaltchuk-santos2  | |
[1] ,Universidade Federal do Rio Grande do Sul Departamento de Genética Porto Alegre RS ,Brazil | |
关键词: Anther culture; soybean; androgenesis; symmetrical mitosis; temperature shock; stress; | |
DOI : 10.1590/S1516-89132004000400006 | |
来源: SciELO | |
【 摘 要 】
In this study the effect of cold and heat shock on androgenetic induction in soybean anther culture was tested. Anthers of soybean were subjected to 4 and 33ºC, while the anthers used for control were maintained at 25ºC. Cytological analysis done during the 30 days of culture showed that frequencies of symmetrical binucleated and multicellular pollen grain did not differ among temperature treatments. Multicellular pollen grain could be formed by symmetrical division, as well as by assymetrical division. In relation to the embryo induction, the results of the treatments did not differ too. These results suggested that these treatments did not induce a sporophytic pathway in soybean.
【 授权许可】
CC BY-NC
All the contents of this journal, except where otherwise noted, is licensed under a Creative Commons Attribution License
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