Effect of temperature shock on soybean microspore embryogenesis

Brazilian Archives of Biology and Technology

Ana Paula De Moraes²  Maria Helena Bonadese-zanettini²  Sídia Maria Callegari-jacques¹  Eliane Kaltchuk-santos² 

[1] ,Universidade Federal do Rio Grande do Sul Departamento de Genética Porto Alegre RS ,Brazil

关键词: Anther culture;    soybean;    androgenesis;    symmetrical mitosis;    temperature shock;    stress;   

DOI  :  10.1590/S1516-89132004000400006

来源: SciELO

【 摘 要 】

In this study the effect of cold and heat shock on androgenetic induction in soybean anther culture was tested. Anthers of soybean were subjected to 4 and 33ºC, while the anthers used for control were maintained at 25ºC. Cytological analysis done during the 30 days of culture showed that frequencies of symmetrical binucleated and multicellular pollen grain did not differ among temperature treatments. Multicellular pollen grain could be formed by symmetrical division, as well as by assymetrical division. In relation to the embryo induction, the results of the treatments did not differ too. These results suggested that these treatments did not induce a sporophytic pathway in soybean.

【 授权许可】

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