Bacterial adherence to different inert surfaces evaluated by epifluorescence microscopy and plate count method

Brazilian Archives of Biology and Technology

Silvânia Quintão Fontes Parizzi¹  Nélio José De Andrade¹  Cleuber Antonio De Sá Silva¹  Nilda De Fátima Ferreira Soares¹  Eldo Antônio Monteiro Da Silva¹ 

[1] ,Universidade Federal de Viçosa Departamento de Tecnologia de Alimentos Viçosa,Brazil

关键词: Bacterial adherence;    Listeria innocua and Staphylococcus aureus;    stainless steel;    polycarbonate and polypropylene;   

DOI  :  10.1590/S1516-89132004000100011

来源: SciELO

【 摘 要 】

The adherence of Listeria inoccua L6a and Staphylococcus aureus ATCC6538 was evaluated on stainless steel (SS), polycarbonate (PC) and polypropylene (PP) chips. The EP results showed a significant difference (p<0.05) among the microorganism species interactions, the surfaces and the contact time. The number of adhered cells on the chip surfaces increased as contact time increased and the number of L. innocua or S. aureus on the surface of SS, PC and PP chips reached 1.0x10(5) CFU/cm² after 12 h of contact, in both methods. The CP method showed a better sensitivity to detect low number of adhered cells. The EP was better when the average number of adhered cells was between 10 and 100 per microscopy field.

【 授权许可】

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