Scale-up of dextransucrase production by Leuconostoc mesenteroides in fed batch fermentation

Brazilian Archives of Biology and Technology

Georgina L. Michelena¹  Aidín Martínez¹  Antonio Bell¹  Emilia Carrera¹  Roxana Valencia¹ 

[1] ,Cuban Institute for Research on Sugar cane By-productsHavana,Cuba

关键词: Leuconostoc mesenteroides;    dextransucrase enzyme;    scale-up;    oxygen transfer;    fed batch fermentation;   

DOI  :  10.1590/S1516-89132003000300017

来源: SciELO

【 摘 要 】

Fed batch fermentation was carried out for the dextransucrase enzyme production from Leuconostoc mesenteroides and the production was scale-up using oxygen transfer criteriuom. It was found that in 5 L vessel fermentation capacity, the best agitation speed was 225 min-1 and aeration rate was 0.15 vvm, obtaining dextransucrase activity of 127 DSU/mL.. The maximum enzyme production velocity coincide with the maximum growth velocity between 6 and 7 h of fermentation, which confirmed that dextransucrase production was associated with microbial growth. High enzyme yields were achieved during scale up based on oxygen transfer rate.

【 授权许可】

CC BY-NC   
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