Improvement of xylanase production by a parasexual cross between Aspergillus niger strains

Brazilian Archives of Biology and Technology

Octavio Loera²  Jesús Córdova¹ 

[1] ,Universidad Autónoma Metropolitana Departamento de Biotecnología México D. F. ,Mexico

关键词: Carbon repression;    xylanase production;    mutants;    parasexual cross;   

DOI  :  10.1590/S1516-89132003000200006

来源: SciELO

【 摘 要 】

A diploid strain (D4) isolated via parasexual recombination between two Aspergillus niger xylanase overproducing mutants was characterised in terms of enzyme production and catabolite repression by glucose. This strain increased xylanase production (607 nkat/ml), which was nearly 100% higher than titers achieved by the wild type strain (305 nkat/ml) and 28% higher than the best mutant used to induce parasexual cycle. Diploid D4 was also less sensitive to carbon catabolite repression by glucose, since xylanolytic activity was detected under conditions normally repressing production by the wild type strain. No decrease in maximal xylanase levels was observed in the presence of glucose for diploid D4.

【 授权许可】

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