Transient gene expression of beta-glucuronidase in citrus thin epicotyl transversal sections using particle bombardment

Brazilian Archives of Biology and Technology

João C. Bespalhok Filho¹  Adilson K. Kobayashi¹  Luiz F. P. Pereira¹  Rafaelo M. Galvão¹  Luiz G. E. Vieira¹ 

[1] ,Instituto Agronômico do Paraná Laboratório de Biotecnologia Vegetal Área de Melhoramento e GenéticaLondrina PR ,Brazil

关键词: Transformation;    uidA expression;    biolistics;    transgenic citrus;   

DOI  :  10.1590/S1516-89132003000100001

来源: SciELO

【 摘 要 】

Studies were carried out to optimize the conditions for transient gene expression through particle bombardment on Carrizo citrange (Citrus sinensis x Poncirus trifoliata) thin epicotyl sections. The best conditions for transient GUS expression were: M-25 tungsten particles, 1550 psi helium pressure, 9 cm distance between specimen and DNA/particle holder and culture of explants in a high osmolarity medium (0.2 M mannitol + 0.2 M sorbitol) 4 h prior and 20 h after bombardment. Under these conditions, an average of 102 blue spots per bombardment (20 explants/plate) were achieved. This protocol is currently being used for transformation of Carrizo citrange and sweet orange (Citrus sinensis).

【 授权许可】

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