The effect of concanavalin A on the replication of rotavirus (SA-11) in cell culture

Brazilian Archives of Biology and Technology

Beatriz S. Hasenack²  Maria Valéria J. Botelho²  Flávio Lauretti²  Fernando L. De Melo²  Janaina M. Orlandi²  Rosa Elisa C. Linhares²  Marli Ueda¹  Carlos M. Nozawa² 

[1] ,Universidade Estadual de Londrina CCB Departamento de MicrobiologiaLondrina Pr ,Brasil

关键词: Rotavirus;    Concanavalin A;    Cell culture;   

DOI  :  10.1590/S1516-89132002000200003

来源: SciELO

【 摘 要 】

Rotavirus (RV) strain SA-11 was studied with respect to its infectivity in MA-104 cell cultures and the effect of concanavalin A (ConA). Receptors for ConA at the surface of MA-104 cell were determined by fluorescence assay and specifically inhibited by D-mannose. The kinetics of virus growth was carried out by plaque assay. Electron microscopy and polyacrylamide gel electrophoresis were used for monitoring the experiments. It was concluded that RV replication was not affected consistently by ConA, however it interfered with the development of cytopathic effect (CPE) without altering virus yields.

【 授权许可】

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