Somatic Embryogenesis in Parana Pine (Araucaria angustifolia (Bert.) O. Kuntze)

Brazilian Archives of Biology and Technology

André Luis Wendt Dos Santos²  Vanildo Silveira²  Neusa Steiner²  Mário Vidor¹  Miguel Pedro Guerra² 

[1] ,Universidade Federal de Santa Catarina Centro de Ciências Agrárias Departamento de FitotecniaFlorianópolis SC ,Brazil

关键词: Araucaria angustifolia;    conifer;    somatic embryogenesis;    Picea abies;    embryo maturation;    Pinus sp.;   

DOI  :  10.1590/S1516-89132002000100015

来源: SciELO

【 摘 要 】

Embryogenic cultures of Araucaria angustifolia were induced from dominant and non-dominant zygotic embryos excised from immature seeds proceeding from three different genotypes and five harvest dates. Zygotic embryos were inoculated in inductive culture medium LP and BM supplemented with or without plant growth regulators 2,4-D (5 µM), BA (2 µM) and Kin (2 µM). The genotype of the mother tree and the developmental explant stage affected the induction frequency. In the maintenance phase, embryogenic cultures were maintained at continuous repetitive cell cycles every 20 days in semi-solid or liquid medium. In the maturation phase the culture medium was supplemented with different types and levels of growth regulators, osmotic agents, carbohydrates and derived. Embryogenic cultures inoculated in culture medium supplemented with PEG 3350 (6 and 9%), maltose (6 and 9%), plus BA and Kin (1 µM each) resulted in the progression of somatic embryos to globular and torpedo developmental stages.

【 授权许可】

CC BY-NC   
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