Bioaccumulation of silver and the isolation of metal-binding protein from P.diminuta

Brazilian Archives of Biology and Technology

Zaharah Ibrahim¹  Wan Azlina Ahmad¹  Abu Bakar Baba¹ 

[1] ,Universiti Teknologi Malaysia Faculty of Science Department of ChemistrySkudai Johor ,Malaysia

关键词: Bioaccumulation;    metal-binding proteins;    Pseudomonas diminuta;   

DOI  :  10.1590/S1516-89132001000300001

来源: SciELO

【 摘 要 】

A silver uptake study by Pseudomonas diminuta was carried out by growing the bacteria in a chloride-free medium (CFM) containing silver ions (50 muM) in a batch culture. From the results, it was found that higher amounts of silver were accumulated inside the cell during early exponential phase compared to the amount bound at the cell surface. This suggested a possible mechanism for metal uptake during bacterial growth. In view of this, attempts were made to isolate proteins which might be associated with silver-binding properties from cultures of P.diminuta grown in the presence and absence of silver. The proteins were first extracted from the bacterial cultures by precipitation with ammonium sulfate followed by purification using isoelectric focussing and SDS-PAGE. Results of the experiment showed the presence of low molecular weight and high molecular weight proteins containing silver with pI values ranging from 2.0 to 9.0 for bacteria grown in the presence of silver.

【 授权许可】

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