Activity determination of Na+ K+ - ATPase and Mg++ - ATPase enzymes in the gill of Poecilia vivipara (Osteichthyes, Cyprinodontiformes) in different salinities

Brazilian Archives of Biology and Technology

Marcelo Da Cunha Amaral²  Ana Cristina Teixeira Bonecker²  Cláudio H. D. Ortiz¹ 

[1] ,Universidade Federal do Rio de Janeiro Instituto de Biologia Departamento de ZoologiaRio de Janeiro RJ

关键词: Poecilia vivipara;    Na+ K+ ATPase;    Mg++ ATPase;    osmoregulation;   

DOI  :  10.1590/S1516-89132001000100001

来源: SciELO

【 摘 要 】

This work aimed to know the tolerance mechanisms through the salinity variation by Na+ K+ - ATPase and Mg++ - ATPase and enzymes encountered in the gills of Poecilia vivipara. In field, the presence of this species was observed in salinities of 0 and 28‰. In laboratory, these fish were maintained in aquarium with mean salinity of 30‰ and positive responses were obtained. Some adult specimens, collected in a lagoon of the Coqueiros Beach, were utilized as matrixes. In the experiments the specimens used were those born in the test aquarium. For each salinity studied three replicates were made with three specimens for each one. The alevins were maintained in salinities of 5, 10, 15, 20, 25, 30 and 35‰ during a month for adaptation. Gills were extracted in appropriate buffer for isolation of plasma membrane and used for specific dosage of the total enzymatic activity of Na+ K+ - ATPase and Mg++ - ATPase. The relation of alevins to their adaptation towards the salinity variation was also studied. The activity of the two enzymes showed a different result. The major expression of Na+ K+ - ATPase was observed in 20‰ (35 µmoles Pi.mg protein.h-1), the best salinity to cultivate P. vivipara.

【 授权许可】

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