Modeling gallic acid production rate by empirical and statistical analysis

Brazilian Archives of Biology and Technology

Bratati Kar²  Rintu Banerjee²  Bimal Chandra Bhattacharyya¹ 

[1] ,Indian Institute of Technology Agricultural & Food Engineering Department Microbial Biotechnology & Downstream Processing LaboratoryKharagpur,India

关键词: Gallic acid;    modeling;    tannase;    Caesalpinia digyna;   

DOI  :  10.1590/S1516-89132000000500010

来源: SciELO

【 摘 要 】

For predicting the rate of enzymatic reaction empirical correlation based on the experimental results obtained under various operating conditions have been developed. Models represent both the activation as well as deactivation conditions of enzymatic hydrolysis and the results have been analyzed by analysis of variance (ANOVA). The tannase activity was found maximum at incubation time 5 min, reaction temperature 40ºC, pH 4.0, initial enzyme concentration 0.12 v/v, initial substrate concentration 0.42 mg/ml, ionic strength 0.2 M and under these optimal conditions, the maximum rate of gallic acid production was 33.49 mumoles/ml/min.

【 授权许可】

CC BY-NC   
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