Compositional changes of proteins and amino acids in germinating coffee seeds

Brazilian Archives of Biology and Technology

Milton Massao Shimizu¹  Paulo Mazzafera¹ 

[1] ,Universidade Estadual de Campinas Instituto de Biologia Departamento de Fisiologia VegetalCampinas SP ,Brazil

关键词: Amino acids;    Coffea arabica;    endosperm;    germination;    protein;    seeds;   

DOI  :  10.1590/S1516-89132000000300003

来源: SciELO

【 摘 要 】

Endosperm is the main reserve tissue in coffee seeds. Coffee (Coffea arabica L.) seeds were germinated for six weeks and qualitative and quantitative changes in amino acids and proteins were investigated. The total content of free amino acids were reduced during germination, however, protein content remained constant. SDS-PAGE profiles showed that legumin-like proteins became less stained in the last weeks. Asparagine, glutamic acid, aspartic acid, alanine and lysine were the major free amino acids, although serine and glutamine were also significant. Except for tyrosine, which increased with germination, all other amino acids were reduced. Analysis of the amino acid composition of the total soluble protein showed glutamic acid/glutamine and glycine as the main amino acids. However, other amino acids such as leucine, aspartic acid/asparagine, alanine, lysine, serine were also found in reasonable amounts.

【 授权许可】

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