Chimeric ß-glucosidase between Cellvibrio gilvus and Ruminococcus albus enzymes

Brazilian Archives of Biology and Technology

Truong Thi Hoa²  Ajay Singh²  Kenji Iwakiri²  Chika Aoyagi²  Ken Tokuyasu²  Kunio Ohmiya¹  Kiyoshi Hayashi² 

[1] ,National Food Research InstituteTsukuba,Japan

关键词: Chimeric;    Cellvibrio gilvus;    Ruminococcus albus;    ß-glucosidase;   

DOI  :  10.1590/S1516-89131999000300006

来源: SciELO

【 摘 要 】

Based on homology search in the amino acid sequences, a chimeric ß-glucosidase with enzyme activity has been prepared by replacing C-terminal region of Thr509-Arg646 from Cellvibrio gilvus with N-terminal region of Arg160-Gly283 from Ruminococcus albus. The chimeric enzyme showed broader optimum pH and slightly higher heat stability compared with original C. gilvus enzyme.

【 授权许可】

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