期刊论文详细信息
Brazilian Archives of Biology and Technology
Chimeric ß-glucosidase between Cellvibrio gilvus and Ruminococcus albus enzymes
Truong Thi Hoa2  Ajay Singh2  Kenji Iwakiri2  Chika Aoyagi2  Ken Tokuyasu2  Kunio Ohmiya1  Kiyoshi Hayashi2 
[1] ,National Food Research InstituteTsukuba,Japan
关键词: Chimeric;    Cellvibrio gilvus;    Ruminococcus albus;    ß-glucosidase;   
DOI  :  10.1590/S1516-89131999000300006
来源: SciELO
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【 摘 要 】

Based on homology search in the amino acid sequences, a chimeric ß-glucosidase with enzyme activity has been prepared by replacing C-terminal region of Thr509-Arg646 from Cellvibrio gilvus with N-terminal region of Arg160-Gly283 from Ruminococcus albus. The chimeric enzyme showed broader optimum pH and slightly higher heat stability compared with original C. gilvus enzyme.

【 授权许可】

CC BY-NC   
 All the contents of this journal, except where otherwise noted, is licensed under a Creative Commons Attribution License

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