Biolistic-mediated transient gene expression in shoot apical meristems of the prickly-pear (Opuntia ficus-indica)

Brazilian Archives of Biology and Technology

Romulo Marino Llamoca-zárate²  Luiz Ferreira Aguiar Ponte²  Joerg Landsmann¹  Francisco De Assis Paiva Campos² 

[1] ,Federal University of Ceará Department of Biochemistry and Molecular Biology Fortaleza Ceará ,Brazil

关键词: Opuntia ficus-indica;    prickly-pear;    Cactaceae;    transient gene expression;    particle bombardment;   

DOI  :  10.1590/S1516-89131999000300005

来源: SciELO

【 摘 要 】

We have demonstrated the transient expression of the GUS gene in cells of the meristematic apical dome of Opuntia ficus-indica. DNA delivery into the cells was achieved using a biolistic PDS-1000He instrument from Bio-Rad Laboratories. The transforming DNA was coated in tungsten particles with diameter of 1.3 m m and the distance between the flying disk and the target tissue was 7.5cm and the shooting pressure was adjusted to 1200 psi. This is the first demonstration that the biolistic transformation system can be used to express a transgene in a member of the Cactaceae.

【 授权许可】

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