Platelet aggregation and quality control of platelet concentrates produced in the Amazon Blood Bank

Revista Brasileira de Hematologia e Hemoterapia

Maria José Dantas Coêlho²  Taysa De Castro Monteiro¹  Felicien Gonçalves Vasquez¹  Kátia Luz Torres Silva¹  Kleber Sandro Brasil Dos Santos²  Viviana Maria Araújo De Oliveira¹  Francimary De Oliveira Cavalcante² 

[1] ,Fundação de Hematologia e Hemoterapia do Amazonas Blood Cycle Department Laboratório de FracionamentoManaus AM ,Brazil

关键词: Hemostasis;    Quality control;    Collagen;    Blood component transfusion;    Blood banks;    Platelet aggregation;   

DOI  :  10.5581/1516-8484.20110030

来源: SciELO

【 摘 要 】

BACKGROUND: The study of platelet aggregation is essential to assess in vitro platelet function by different platelet activation pathways. OBJECTIVE: To assess aggregation and biochemical parameters of random platelet concentrates produced at the Fundação HEMOAM using the quality control tests defined by law. METHODS: Whole blood samples from 80 donors and the respective platelet concentrate units were tested. Platelet concentrates were tested (platelet count, aggregation and pH) on days 1, 3 and 5 of storage. Additionally a leukocyte count was done only on day 1 and microbiological tests on day 5 of storage. Collagen and adenosine diphosphate were used as inducing agonists for platelet aggregation testing. RESULTS: Donor whole blood had normal aggregation (aggregation with adenosine diphosphate = 67% and with collagen = 78%). The median aggregation in platelet concentrates with adenosine diphosphate was low throughout storage (18% on day 1, 7% on day 3 and 6% on day 5) and the median aggregation with collagen was normal only on day 1 and low thereafter (54.4% on day 1, 20.5% on day 3 and 9% on day 5). CONCLUSION: Although the results were within the norms required by law, platelet concentrates had low aggregation rates. We suggest the inclusion of a functional assessment test for the quality control of platelet concentrates for a more effective response to platelet replacement therapy.

【 授权许可】

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