Genetic diversity of the Brazilian Creole cattle Pé-duro assessed by microsatellites and mitochondrial DNA

Revista Brasileira de Zootecnia

Ana Paula Ferreira De Oliveira²  José Herculano De Carvalho¹  Marcos Mateo Miretti¹  Maria Aparecida Cassiano Lara¹  Eucleia Primo Betioli Contel² 

[1] ,Universidade de São Paulo Faculdade de Medicina de Ribeirão Preto Departamento de Genética SP ,Brazil

关键词: Bos taurus;    cattle;    conservation;    genetic diversity;    microsatellites;    mitochondrial DNA;   

DOI  :  10.1590/S1516-35982012001100003

来源: SciELO

【 摘 要 】

The objective of this study was to describe the genetic diversity and structure of the largest Pé-duro population by assessing variation at ten autosomal microsatellite (STR) loci and mitochondrial DNA (mtDNA) sequences. The mean expected heterozygosity was 0.755, the mean observed heterozygosity was 0.600 and significant inbreeding coefficient (Fis) and deviations from the Hardy-Weinberg equilibrium in most of analyzed loci demonstrate the impact of inbreeding and homozygosis on this population. A more in-depth genetic analysis could be achieved by expanding the STR list. The analysis of mtDNA provided evidence of ancestral African taurine haplotypes in Pé-duro and excluded maternal Zebuine introgression. In this report, the main Pé-duro population is genetically portrayed by sampling approximately 40% of it. As this herd represents the core of the Pé-duro conservation program, these findings are of outstanding value for the management and preservation of this Brazilian 'native' cattle breed.

【 授权许可】

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